UNSPSC Code: 41116133
Components: PCR DIG Labeling Mix, containing 2 mM dATP, dCTP, dGTP, 1.9 mM dTTP, and 0.1 mM Digoxigenin-dUTP; PCR Reaction Buffer, without MgCl2, 100 mM Tris/HCl, 500 mM KCl, pH 8.3 (20 °C) 10x concentrated; MgCl2 Stock Solution 25 mM; PCR Buffer, 100 mM Tris/HCl, 15 mM MgCl2, 500 mM KCl, pH 8.3 (20 °C) 10x concentrated; Taq DNA Polymerase, in storage buffer: 20 mM Tris/HCl, 1 mM dithiothreitol, 0.1 mM EDTA, 0.5% Nonidet P40 (v/v), 50% Tween 20 (v/v), 0.5% glycerol (v/v), pH 8.0 (+4 °C) 5 U/μl; Control PCR Primer Mix, specific for the human t-PA gene 125 pmol each; Human Control DNA 1 ng/μl; Water, PCR Grade
RIDADR: NONH for all modes of transport
The kit is used for nonradioactive labeling of DNA by PCR. For detection and quantification of the PCR product, we recommend to you use the PCR ELISA, DIG Detection Kit. The PCR ELISA allows the sensitive and specific detection of PCR products in solution. It is approximately 100 times more sensitive than conventional ethidium bromide-stained agarose gels. The convenient ELISA format makes this kit particularly suitable for determining the presence or absence of a specific target sequence in a large number of samples. The use of a target-specific capture probe allows differentiation between products that differ by a single base pair. Thus, the kit can also be used for the detection of point mutations, deletions, or insertions. The microplate format combined with colorimetric detection allows quantification of the amount of template present in a sample when used in combination with suitable standards. When using appropriate capture probes, the kit can be used for the typing of a target sequence (e.g., in HLA-typing or cell typing). The microplate format makes this kit particularly suited for automation.
The kit has been used in RT-PCR ELISA method to measure the dihydropyrimidine dehydrogenase (DPD) mRNA expression in liver and cell cycle phase distribution of bone marrow cells.
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.Â The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
For life science research only. Not for use in diagnostic procedures.
For best results run a negative control omitting template DNA to check for cross contamination from reagents.