This is the Hot-Start Blue version of Choice-Taq™Blue DNA Polymerase that requires a pre-activation step at 95°C for 5 minutes in order to achieve a fully functional polymerase enzyme. The inactivity of the enzyme at room temperature increases the specificity of the enzyme for the desired template.
Hot-Start Taq Blue DNA Polymerase is supplied with an inert blue dye that allows the end user a method to confirm addition of the enzyme to the reaction. The dye further allows the user to directly add a post-reaction sample directly into an agarose gel, eliminating the need for a separate gel-loading buffer.