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ACTGene, Inc.

ACTaq™ High Fidelity DNA Polymerase

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ACTaq™ High Fidelity DNA Polymerase, originating from Pyrococcus furiosus with molecular weight of 90KDa, is a thermo stable enzyme and exhibits the lower error rate of DNA amplification. For use in DNA fragment extension amplification reactions with 3' to 5' exonuclease proofreading activity; at extension amplification speed of 1000 bases per minute and optimized between 65-75oC, when using dNTP concentration at 100-300µM, Mg2+ concentration at 2.0-3.0mM, and pH at 8.1-9.1. ACTaq™ High Fidelity DNA Polymerase generates blunt-ended fragment.

Storage Condition:-20°C. Stable for at least 12 months in constant freezer temperature
Concentration:5 Units/μL
Unit Definition:One unit of the amount of enzyme that incorporates 10nmols of dNTPs into acid-insoluble material in 30 minutes at 72°C
Product Components:ACTaq™ High Fidelity DNA Polymerase, 10x PCR reaction buffer with Mg2+
Storage Buffer:10mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Tween® 20, 0.5% NP-40, 50% Glycerol
PCR Reaction Buffer:10x PCR buffer with Mg2+. 20mM MgSO4, 100mM KCl, 160mM
(NH4)2SO4, , 200mM Tris-HCl, pH 8.8, 0.5% Triton X100, 1mg/ml BSA.
Quality Control:Activity of PCR for 1Kb fragment. Free of contaminating endonucleases, exonucleases, and nicking activities. The purity of the enzyme is evaluated by SDS-PAGE at >95% purity. BSA, 0.5% NP-40, 50% glycerol

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Product Detail
Thomas No.
C995K16
Mfr. No.
E2000-250U
Also known as: CB4085-HF, DNP-E200
Description
ACTaq™ High Fidelity DNA Polymerase, 250Units/Vial
list price/quantitytotal
$0.00
Thomas No.
C995K17
Mfr. No.
E2000-1000U
Description
ACTaq™ High Fidelity DNA Polymerase, 1000Units/Vial
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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