Application
The PCR Nucleotide Mix is optimized for use in all types of amplification reactions and primer-extension reactions:
- PCR
- RT-PCR
- cDNA synthesis
- Primer extension
Features and Benefits
The PCR Nucleotide Mix can be added directly to amplification reactions. PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity.
Improve yield and performance
- Choose a convenient ready-to-use mix of all 4 nucleotides.
- Achieve the highest PCR and RT-PCR sensitivity.
- Insist on highest purity (>99%) nucleotides.
General Description
PCR Nucleotide Mix is a clear, colorless solution of the sodium salts of dATP, dCTP, dGTP, and dTTP, each at a concentration of 10 mM in Water, PCR Grade. This nucleotide mixture can be added directly to polymerase chain reactions.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Packaging
1 set containing 4 ready-to-use mixes
Preparation Note
Activator: Mg2+
Working solution: If required, the solutions can be diluted with distilled autoclaved water.
Storage conditions (working solution): It will withstand 50 freeze/thaw cycles.
Quality
Function Testing in PCR
Function Testing in RT-PCR
Absence of Contaminating Deoxyribonucleases/Nicking Activities according to the current Quality Control procedures.
Absence of Contaminating Ribonucleases according to the current Quality Control procedures.
Form: solution
Usage: sufficient for 500 reactions (11581295001)
Packaging: pkg of 200 μL (11581295001 [10 mM, each])