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MP Biomedicals

Histopaque™ 1077

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Histopaque™1077 is a solution of polysaccharide (Ficoll-400) and a radiopaque contrast medium (sodium diatrizoate).Low viscosity of Ficoll® is used for lymphocyte isolation procedure involving a short, low speed centrifugation. A solution of Ficoll® and sodium metrizoate of the proper density and osmotic strength is placed in a centrifuge tube. Blood is layered on top, and the two-phase system was centrifuged at a low speed for a short time. The erythrocytes and granulocytes sediment to the bottom of the tube. Because of lower density, the lymphocytes are found at the interface between the plasma and the Ficoll-Paque with other slowly sedimenting particles (platelets and monocytes). The ficoll-sodium metrizoate centrifugation procedure is an easy one-step, rapid, reproducible method for the preparation of viable lymphocytes in high yield. Sodium diatrizoate can be substituted as sodium metrizoate.

Ficoll is used in isolating lymphocytes from whole blood in areas such as histocompatability testing, in vitro cell-mediated immunity assays, and other procedures requiring pure lymphocyte preparations. Since this method can be adapted to very small volumes of blood, it is particularly suitable for isolation of lymphocytes where only limited quantities of blood are available, such as from cadavers and small children. Because of the rapidity and simplicity of the method, it is also the method of choice in emergency tissue typing procedures.

Product Type:Biochemicals
Biochemical Category:Cell Seperation Media
Density:1.077 g/mL
Presentation:Ficoll-Paque is supplied as a sterile solution in bottles of 100 mL.
Formulation:Each 100 mL contains Ficoll® 400, 5.7g, Diatrizoate Sodium, 9.0 g, with Edetate Calcium Disodium in Water for Injection U.S.P.
Sterility:Sterile
Storage & Handling:Store at +4 °C,Protect from light.

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Product Detail
Thomas No.
C833U18
Mfr. No.
0219083780
Description
Histopaque™ 1077 100mL
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product C833U18 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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