EMM medium (Edinburgh Minimal Medium) is a minimal defined medium for the growth of Schizosaccharomyces pombe.
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Reagents are tested and chosen for their ability to promote optimum growth of S. pombe when combined in EMM medium formulations. Chosen reagents are combined in the proper proportions according to the EMM formula and milled to a powder of consistent sized particles. A sample of the milled formulation was used to prepare liquid and agar medium by adding 32.2 g powder per liter of purified water. For agar medium 17g/L of agar-Y was added. The combinations were mixed by hand for about 1 minute to dissolve the glucose and 0.25g/L of a mixture of adenine, his, leu, ura, and lys was added. Autoclaving is not recommended as it may cause precipitation. Sterile filter the solution into a sterile bottle.