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Agilent Genomics

pCMV-Script Vectors

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pCMV-Script vectors are mammalian expression vectors for efficient cloning of PCR fragments.

CMV promoter for high-level protein expression in mammalian cells

Large multiple cloning site contains 15 unique sites

Clone PCR products generated with any PCR enzyme

The pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1 hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.

The pCMV-Script vector is derived from a high-copy-number pUC-based plasmid and is designed to allow protein expression in mammalian systems. Mammalian expression is driven by the human cytomegalovirus (CMV) immediate early promoter to promote constitutive expression of cloned inserts in a wide variety of cell lines. Selection is made possible in bacteria by the kanamycin-resistance gene under control of the prokaryotic ß-lactamase promoter. The neomycin-resistance gene is driven by the SV40 early promoter, which provides stable selection with G418 in mammalian cells. 

This product is only available to U.S. Domestic Customers.

For research use only, not to be used in diagnostic procedures.

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Product Detail
Thomas No.
CHM01R073
Mfr. No.
212220
Pkg. Size
20 µg
UN #
UN1845
Description
pCMV-Script Vector 20 µg, pCMV-Script vector (supercoiled), XL1-Blue MRF' host strain (undigested)

Domestic Only (Domestic Only)

list price/quantitytotal
$0.00
Thomas No.
CHM01R074
Mfr. No.
212224
Pkg. Size
55 Rxn
UN #
UN1845
Description
pCMV-Script XR Predigested Vector 55 Rxn, pCMV-Script vector (digested with Xho I and EcoR I), test insert

Domestic Only (Domestic Only)

list price/quantitytotal
$0.00
$0.00 (0 Items)
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