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Agilent Genomics

TaqPlus Precision PCR System

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The TaqPlus Precision PCR system exhibits the highest replication accuracy of any mixture.

2.7 times more accurate than Taq DNA polymerase

Optimized blend of Pfu and Taq2000 DNA polymerases;Amplifies templates up to 15 kb

The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.

Thermostable DNA polymerases offer distinct advantages for specific PCR applications. Pfu DNA polymerase exhibits the lowest error rate of any thermostable DNA polymerase analyzed. This proofreading enzyme is also characterized by a relatively slow polymerization rate requiring the use of long extension times (i.e., ³2 minutes/kb of DNA target) to generate high yields of PCR product. Taq DNA polymerase exhibits a fivefold higher polymerization rate than Pfu DNA polymerase and is used routinely to generate high yields of PCR product £5 kb in length using short extension times (i.e., ~1 minute/kb of DNA target). Taq DNA polymerase possesses a significantly higher error rate than Pfu DNA polymerase. However, Taq2000 DNA polymerase is superior to other commercial Taq DNA polymerases in that this recombinant version of Taq DNA polymerase minimizes artifactual smearing in PCR amplification reactions requiring long extension times.

DNA polymerase mixtures, consisting of a non-proofreading DNA polymerase and a proofreading DNA polymerase, are typically employed to amplify longer DNA targets and to generate higher yields of PCR product than can be obtained using either DNA polymerase alone. Agilent offers two mixtures of Taq and Pfu DNA polymerases designed for PCR: the TaqPlus Long PCR system and the TaqPlus Precision PCR system. The TaqPlus Long PCR system typically generates the highest yields of PCR product and can be used to amplify unusually difficult or long targets (up to 35 kb). The TaqPlus Precision PCR system also generates relatively high yields of PCR product using short PCR extension times. The error rate, however, is significantly lower than the TaqPlus Long PCR system. In addition, the TaqPlus Precision PCR system successfully amplifies plasmid and lambda DNA templates up to 15 kb in length and single-copy genomic DNA templates up to 10 kb in length as well as templates that are difficult to amplify using single enzyme formulations.

This product is only available to U.S. Domestic Customers.

For research use only, not to be used in diagnostic procedures.

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Product Detail
Thomas No.
CHM01R360
Mfr. No.
600210
Pkg. Size
100 U
UN #
UN1845
Description
TaqPlus Precision PCR System 100 U, Accurate PCR with high yields, shorter extension times than Pfu DNA polymerase alone.

Domestic Only (Domestic Only)

list price/quantitytotal
$0.00
Thomas No.
CHM01R361
Mfr. No.
600212
Pkg. Size
1000 U
UN #
UN1845
Description
TaqPlus Precision PCR System 1000 U, Accurate PCR with high yields, shorter extension times than Pfu DNA polymerase alone.

Domestic Only (Domestic Only)

list price/quantitytotal
$0.00
$0.00 (0 Items)
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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