The GNOME™ kit utilizes RNase Mixx to eliminate RNA immediately after lysis, and Protease Mixx to rapidly degrade cellular proteins. This is followed by a proprietary “salting-out” technique which precludes the need for phenol, chloroform, or other organic extractions. The DNA can either be spooled, removed with forceps, or centrifuged and dissolved in TE.
The GNOME™ DNA Isolation kit is used to quickly and efficiently isolate high molecular weight genomic DNA from cells and tissues of any type, including bacteria, yeast, plant, and animal cells. Each preparation with the GNOME™ kit yields up to 100 µg of genomic DNA. The DNA isolated by the GNOME¨ procedure is suitable for restriction enzyme digestion or PCR amplification in as little as 1 hour after cell lysis.
The yield of genomic DNA and the speed of the protocol are greatly enhanced through the use of the FastPrep™ System