Key to the kit is our proprietary DNA binding systems that allow the highly efficient binding of DNA to our ezBind™ matrix while proteins and other impurities are removed by Wash Buffer. Nucleic acids are easily eluted with sterile water or Elution Buffer.
Unlike other kits in the markets, no chaotropic salts are contained in the buffer of our patented plasmid purification kit. The purified DNA is guanidine/anion exchange resin residues free.
This kit is designed for fast and efficient purification of plasmid DNA from 100 to 200 mL of E. coli culture. The midi column has a plasmid DNA binding capacity of 1000 µg.
The purified DNA is ready for high performance of downstream applications such as transfection of robust cells such as HEK293, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.