The innuSPEED Bacteria/Fungi DNA Kit has been specially developed for isolating genomic DNA from gram+ bacteria, fungal spores and yeasts. The extraction protocol combines two steps: rapid homogenization of these hard-to-access starting materials, and efficient digestion of microbial cell walls in specially developed Lysis Tubes (using a SpeedMill or other homogenizer). The material is first broken down mechanically and then subjected to thermal and enzymatic lysis. The DNA is then selectively bound to the surface of to remove inhibitors, and then by addition of a low-salt buffer to separate bound DNA from the membrane.
Features
- Optimum isolation of genomic DNA from gram+ bacteria, yeast and fungal spores
- Includes Lysis Tubes with application-specific beads for highly efficient homogenization with a SpeedMill (or other homogenizers)
- Effective sample disruption for extremely high DNA yields
- High-quality DNA; free of inhibitors