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Analytik Jena

innuSPEED Bacteria/Fungi DNA Kit

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The innuSPEED Bacteria/Fungi DNA Kit has been specially developed for isolating genomic DNA from gram+ bacteria, fungal spores and yeasts. The extraction protocol combines two steps: rapid homogenization of these hard-to-access starting materials, and efficient digestion of microbial cell walls in specially developed Lysis Tubes (using a SpeedMill or other homogenizer). The material is first broken down mechanically and then subjected to thermal and enzymatic lysis. The DNA is then selectively bound to the surface of to remove inhibitors, and then by addition of a low-salt buffer to separate bound DNA from the membrane.

Features

  • Optimum isolation of genomic DNA from gram+ bacteria, yeast and fungal spores
  • Includes Lysis Tubes with application-specific beads for highly efficient homogenization with a SpeedMill (or other homogenizers)
  • Effective sample disruption for extremely high DNA yields
  • High-quality DNA; free of inhibitors
Starting materialGram+ bacteria (max. 1 x  109)
Fungal spores
Yeasts  (max.  1 x  109)
Extraction time
  • Homogenization and lysis: approx. 30 – 45 minutes
  • Purification: approx. 12 minutes
Binding capacityColumn binding capacity: > 30 µg DNA
Average yieldDepends on the type and quantity of the starting material used; up to 30 µg
Average purity1.7 – 2.0

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Product Detail
Thomas No.
C866M67
Mfr. No.
845-KS-1510050
Description
innuSPEED Bacteria/Fungi DNA Kit, 50 reactions
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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