SPINeasy DNA/RNA/Protein All-In-One Kit utilizes a convenient workflow and silica membrane spin columns to isolate DNA, RNA, and protein components from the same sample, without the use of toxic substances such as phenol and chloroform. The use of our specially formulated Lysis Buffer R and Lysing Matrix A in combination with FastPrep instruments from MP Biomedicals enables highly efficient lysis of tissue samples within seconds. DNA, RNA, and proteins are then sequentially purified from the same lysate. Briefly, DNA is absorbed onto the first spin column, while the flow-through is collected and used for RNA purification. The second spin column captures RNA and the flow-through from this step is used for protein extraction. DNA and RNA are bound on the first and second columns, respectively, and then washed and eluted. In the final extraction, proteins are precipitated out of solution, pelleted down by centrifugation, washed, and resuspended. Each molecular component is then immediately available for their respective downstream applications.
The SPINeasy DNA/RNA/Protein All-In-One Kit quickly and high-efficiently isolate DNA, RNA and protein from the same sample, without the use of toxic substances.
Research Use Only (RUO)
PCR, RT-PCR, cDNA synthesis, NGS, Western blots, ELISA , Genomics, Molecular Biology
Key features and details
- Fast and efficient isolation of DNA, RNA and protein from a single sample using spin column technology
- Lysing Matrix A for thorough and complete tissue lysis with FastPrep instruments
- High quality DNA/RNA/protein for multiple downstream applications
Alternate Names: SPINeasy DNA/RNA/Protein Extraction Kit; Multianalyte Extraction Kit
Application Notes: The SPINeasy DNA/RNA/Protein All-In-One Kit quickly and high-efficiently isolate DNA, RNA and protein from the same sample, without the use of toxic substances.
Base Catalog Number: 65440
Components: 50x Lysing Matrix A, 60 mL Lysis Buffer R, 30 mL Wash Buffer D, 12 mL Wash Buffer R, 10 mL Elution Buffer GD, 10 mL Nuclease-free water, 1 vial DNase I, 5 mL DNase I Buffer, 80 mL Protein Precipitant, 100x Column A with collection tube
Hazard Statements: Hazard Class 8
Pack Size: 50 Preps
Personal Protective Equipment: Safety Goggles/Faceshield; Gloves
Sample Type: Blood, Cell Culture, Animal Tissue, Swabs
Storage: Store at Room Temperature (15-25 °C) in a dry environment
Usage Statement: Research Use Only (RUO).