prepGEM Universal is MicroGEM’s comprehensive solution for effortless high-yield DNA extraction from blood, saliva, and tissues. It is also perfectly suited for extracting DNA from cell cultures. All within a single tube, this method ensures no loss of DNA and excels with low cell number extractions. This kit is an excellent tool for researchers performing confirmatory genotyping of transfected cells following CRISPR/Cas9 gene editing and is also suitable for STR, PCR, qPCR, whole genome amplification, and amplicon sequencing. prepGEM Universal utilizes MicroGEM’s thermophilic proteinase activated with a simple temperature change. The whole process is easily automated in a single tube using standard laboratory equipment. A standard thermocycler can be programmed to carry out the extraction.
- Stream-lined approach provides DNA in approximately 15 minutes
- Inhibitor-free solutions mean no harsh chemical washes or multiple steps are required
- Closed-tube extraction protects the integrity of the sample
- Simplified, hands-off workflow frees up technician time, reduces lab costs, and significantly reduces hazardous/plastic waste