JM109 competent cells for routine cloning are recombination- and endonuclease-deficient.
Recombination- and endonuclease-deficient
Blue-white color screening possible for recombinant plasmids
Transformation efficiency 1 x 10^8 transformants/µg DNA
JM109 competent cells are a classic E. coli strain for routine cloning and plasmid maintenance. E. coli K12 JM109 is recombination- and endonuclease-deficient, improving insert stability and quality of miniprep DNA, respectively. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system.
This product is only available to U.S. Domestic Customers.
For research use only, not to be used in diagnostic procedures.