Difficult Cloning Competent Cell Packs include three strains packaged together for lab efficiency.
XL10-Gold ultracompetent cells, with the novel Hte (high transformation efficiency) phenotype, provide 25-fold higher transformation of ligated DNA compared to DH10B and XL2-Blue cells
SURE supercompetent cells clone DNA segments “unclonable" in conventional E. coli strains, with transformation efficiency of 1 x 10^9 transformations/µg DNA
Able K competent cells reduce copy number of ColE1-derived plasmids 10-fold, decreasing the level of cloned gene products and enhancing probability of toxic clone propagation
Difficult Cloning Competent Cell Packs contain three strains packaged together to increase lab efficiency, optimize protocols economically, and test multiple strains simultaneously. Use XL10-Gold Kanr ultracompetent cells to transform large plasmids or work with limited amounts of DNA.
Use SURE supercompetent cells to clone eukaryotic segments of DNA containing inverted repeats or secondary structures, such as Z-DNA. And use Able K competent cells to clone toxic genes present in any ColE1-derived plasmid and isolate sufficient DNA for most downstream applications.
This product is only available to U.S. Domestic Customers.
For research use only, not to be used in diagnostic procedures.