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Omega Bio-tek

E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit II

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Plasmid isolated with traditional purification procedures normally contains high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. Up to 5 mL of overnight cultures can be used for high copy number plasmids and 10 mL for low copy number. The E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit I integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade(<1 eu g) plasmid for efficient transfection. the bacterial cells are lysed using the alkaline-sds lysis method. the cleared cell lysate is then treated with etr reagent to efficiently remove the endotoxins. after adjusting the binding conditions, the cell lysate is applied into the hibind® dna column and purified dna is eluted from the column membrane.<>

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Product Detail
Thomas No.
CHM02C985
Mfr. No.
D6950-00
Description
E.Z.N.A.® Endo-free Plasmid DNA Mini Kit II - 5 Preps
list price/quantitytotal
$0.00
Thomas No.
CHM02C986
Mfr. No.
D6950-01
Description
E.Z.N.A.® Endo-free Plasmid DNA Mini Kit II - 50 Preps
list price/quantitytotal
$0.00
Thomas No.
CHM02C987
Mfr. No.
D6950-02
Description
E.Z.N.A.® Endo-free Plasmid DNA Mini Kit II - 200 Preps
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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