For use in tissue culture media
A broad range, 200X concentrated, ready-to-use protease inhibitor cocktail sterile filtered for tissue culture media. TCM ProteaseArrest™ inhibits a wide range of serine, cysteine and other specific proteases including aminopeptidasestrypsin-like and acid proteases.
The inhibitor cocktail is designed to protect secreted proteins during cell culture for up to 48 hours.
ProteaseArrest Family
Our ProteaseArrest™ is a highly efficient, liquid format general protease inhibitor cocktail that offers >95% protease inhibition.
The ProteaseArrest™ family of inhibitor cocktails includes other species-specific inhibitor cocktails as well, all of which are enhanced with inhibitors specific for that species. In addition to Mammalian, species-specific cocktails are available for Mammalian , Plant , Yeast , Fungus and Bacteria samples.
Our Recom ProteaseArrest™ is designed for recombinant protein purification, particularly 6X His tagged proteins. These proteins are purified by IMAC (Immobilized Metal Affinity Chromatography), which is severely inhibited by the routinely used metalloproteases inhibitor EDTA. Recom ProteaseArrest™ contains a specific bacterial metalloproteases that does not interfere with 6X His protein purification.
Our FOCUS™ ProteaseArrest™ is designed to protect proteins destined for 2D electrophoresis and mass spectrometry analysis.
Features
- Broad range protease inhibitor cocktail
- Stable for up to 48 hours in cell culture media
- Sterile filtered
Applications
- Protect secreted proteins from proteolytic degradation during cell culture