Sample: 200 μl sample (plasma, serum, body fluid or the sup ernatant of viral infected cell cultures)
Format: Spin column
Rxns: 50,100 and 300
Operation time: 40 minutes
IBI’s Viral Nucleic Acid Extraction Kit was designed for purification of viral DNA/RNA from cell-free samples; such as serum, plasma, body fluids, and the supernatant of viral infected cell cultures. DNA/RNA viruses are lysed quickly and efficiently using the lysis buffer, which is a highly concentrated solution of chaotropic salt. When combined with ethanol, the AD Buffer creates an optimum condition for nucleic acid binding to the glass fiber matrix of the column. Contaminants such as salts, metabolites, and soluble macromolecular cellular components are removed in the wash process. Nucleic Acid is eluted in RNase-free water and is then ready for use in subsequent reactions, including Real-Time PCR, Automated Fluorescent DNA Sequencing, PCR, and other enzymatic reactions. The detection limit for certain viruses depends on the sensitivity of the individual PCR or RT-PCR assays.
Kit Contents
| Name | C985G75 | C985G76 | C985G77 | C985G78 |
| VB Lysis Buffer | 2 mL | 30 mL | 60 mL | 130 mL |
| AD Buffer1 | 0.5 mL | 4 mL | 8 mL | 24 mL |
| (Add Ethanol) | (4 mL) | (30 mL) | (60 mL) | (180 mL) |
| W1 Buffer | 2 mL | 30 mL | 50 mL | 130 mL |
| Wash Buffer2 | 1 mL | 12.5 mL | 25 mL | 50 mL |
| (Add Ethanol) | (4 mL) | (50 mL) | (100 mL) | (200 mL) |
| RNase-free Water | 1 mL | 6 mL | 6 mL | 30 mL |
| VB Column | 4 pcs | 5 pcs | 100 pcs | 300 pcs |
| 2 mL Collection Tube | 8 cps | 100 pcs | 200 pcs | 600 pcs |
1Add absolute ethanol (see the bottle label for volume) to the AD Buffer prior to initial use
2Add absolute ethanol (see the bottle label for volume) to the Wash Buffer prior to initial use
Caution
VB Lysis Buffer contains chaotropic salt which is a harmful irritant. During operation always wear a lab coat, disposable gloves and protective goggles.