Quanta Mega Kit is uniquely designed to facilitate ultrapure pDNA (2.5 mg) free from transfection even into highly sensitive mammalian cells. These kits offer high yields from both low copy as well as high copy number plasmids. The innovative buffer system and filter device provides efficient binding of pDNA onto the spin column.
Washing is done with the help of provided wash buffers in order to remove endotoxins, RNA, proteins and polysaccharides. The technology also does away with hasselsome gravitational waiting, phenol extraction (associated with desalting) and ethanol precipitation (associated with anion exchange based purification).
Features
- Fastest Megaprep for pDNA Isolation
- Very high binding capacity - Up to 3000µg
- No gravitational waiting
- Simple filtration to remove cell debris; No centrifugation
- No precipitation required for concentration
- Higher yields with low culture volume
- Highly concentrated, ultrapure pDNA isolation; in very low elution volumes
- High within lot as well as lot to lot consistency
Applications
- Automated Fluorescent Sequencing
- Radioactive Sequencing
- Restriction Digestion
- Transfection (with highly sensitive mammalian cell lines)
- Cloning
- PCR