Ultra-pure and large-volume plasmid preparation
IBI’s MIDI and MAXI Plasmid Kits (Endotoxin Free) use pre-packed, ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be washed off with a wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than 2 hours; and the obtained high purity plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription.