Endotoxin Free Quanta Midi Kit is uniquely designed to facilitate ultrapure pDNA free from endotoxins (<0.1EU/µg), ideal for transfection even into highly sensitive mammalian cells. These kits offer high yields from both low copy as well as high copy number plasmid. The innovative buffer system and filter device provides efficient binding of pDNA onto the spin column.
Features
- Extremely low Endotoxin levels, <0.1 EU/μg
- Fastest pDNA isolation in <30 minutes
- Very high binding capacities and yields in miniaturized spin columns
- Highly concentrated ultrapure pDNA: more than 1μg/μl
- Eluted pDNA is ideal for transfection into highly sensitive mammalian cell lines
- No salt contamination due to isopropanol precipitation
- No yield losses due to DNA reconstitution
- No gravitational waiting
- Simple filtration to remove cell debris; No centrifugation
- High within lot as well as lot to lot consistency
Applications
- Automated Fluorescent Sequencing
- Radioactive Sequencing
- Restriction Digestion
- Transfection (with highly sensitive mammalian cell lines)
- Cloning
- PCR