SIGMA Tris-Borate-EDTA buffer, BioReagent, for molecular biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Synonyms: TBE buffer

Application: Ready for use in gel electrophoresis after dilution to working concentrations.

Preparation Note: Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Reconstitution: Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water.

Packaging: The 1-liter size is packaged in a plastic bottle large enough to contain the 5× concentrate.