Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 40000pg/ml, 20,000pg/ml,10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml mouse MMP-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma (heparin) to each empty well. It is recommended that each mouse MMP-2 standard solution and each sample be measured in duplicate.