INDIGO's Aryl Hydrocarbon Receptor (AhR) Reporter Cells include the luciferase reporter gene functionally linked to an AhR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in AhR activity. The principle application of this reporter assay system is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against human AhR. AhR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spinand-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. INDIGO’s Human AhR assay kit is an all-inclusive system. In addition to AhR Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a reference agonist, Luciferase Detection Reagent, and a cell cultureready assay plate.
- Screen test samples to quantify functional activity, either agonist or antagonist, that they may exert against AhR
- Robust technology provides sustained response for consistent and reproducible data acquistion
- Included in kit: Ready-to-use, pre-transfected reporter cells | Optimized media | Reference agonist | Luciferase detection reagent | Cell culture ready assay plate