MDL No.: MFCD00162107
Storage: -20C
UNSPSC Code: 12352203
General description: alpha-Actinin is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, alpha-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres. Rabbit anti-alpha-actinin antibody stains alpha-actinin in cell cultures using indirect immunofluorescence. The antibody reacts best with cultured chicken fibroblasts, however, adequate labeling may also be obtained with cells of other species. In an immunoblot assay, the antiserum specifically stains the alpha-actinin band.
Application: Anti-Actinin antibody was used to detect actinin in human mesangial cells. Cells were first grown on glass cover slips, fixed in cold methanol and permeabilized with 3.5% paraformaldehyde/0.1% Tween-20 for 5 min at room temperature.
Application: Rabbit anti-α-actinin antibody has been used for co-immunoprecipitation studies. The product has also been used for immunoblotting studies using Arabidopsis tissue extracts.
Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunofluorescence (1 paper)
Immunogen: alpha-actinin from chicken gizzard
RIDADR: NONH for all modes of transport
WGK Germany: 3
R Codes: 21/22
S Codes: 36/37
IOD Codes: Xn