MDL No.: MFCD00162629
UNSPSC Code: 12352203
General description: Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Antibody is isolated from goat anti-mouse IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins that do not specifically bind to the Fc fragment of mouse IgG. Anti-Mouse IgG is conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross reactivity in tissue or cell preparations.Specificity and cross-reactivity of anti-Mouse IgG (Fc Specific)-Peroxidase has been evaluated by ELISA. The conjugate is specific for mouse IgG and mouse IgG Fc fragment and shows no reactivity with mouse Fab fragment or human IgG. Furthermore, the antibody conjugates does not bind other mouse Igs.
Application: Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.
Application: Peroxidase-conjugated goat anti-mouse IgG (Fc specific) antibody was used to detect the level of IgGs in mouse sera following Sv40Tag infection by indirect ELISA at a 1:1000 dilution in 10% normal goat serum for 30 minutes at 37C. Peroxidase-conjugated goat anti-mouse IgG (Fc specific) antibody was used to detect the Sv40Tag mAB in murine breast tumor cell line 4T1 and kidney tumor cell line mKSA by western blot at a dilution of 1:2000 for 2 hours at room temperature. Western blot was developed using 3, 3â€² diamino-benzidine reagent (Sigma) as the substrate.
Immunogen: purified mouse IgG Fc fragment
Physical form: Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal.
Preparation Note: Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
RIDADR: NONH for all modes of transport