Anti-Axin1 (C-terminal region) antibody produced in rabbit, ~2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyms: Anti-Axin; Anti-Axis Inhibitor 1; Anti-Axis inhibition protein 1

MDL No.: MFCD02095870

Storage: -20C

UNSPSC Code: 12352203

General description: Axin1 (axis inhibitor-1 or axin) functions as a negative regulator of Wnt signaling. Axin1 is known to associate with several members of the Wnt pathway such as beta-catenin, glycogen synthase kinase-3b (GSK-3b), dishevelled (Dsh) and adenomatous polyposis coli (APC). Rabbit anti-axin1 (C-terminal) antibody is specific for human and mouse axin1 by immunoblotting (approx. 130kDa). Staining of the axin1 band in immunoblotting is specifically inhibited by the immunizing peptide. The antibody also associates with rat axin1.

Application: Immunohistochemistry was performed using rabbit anti-axin antibody to visualize Axin in the mouse developing cortex. Mouse brains had been previously harvested and fixed in 4% paraformaldhyde and cryoprotected in 20% sucrose. Axin was seen in the soma and axons of neurons.

Application: Rabbit anti-axin1 (C-terminal region) antibody can be used at a concentration of 0.5-1mug/ml in western blot assays using HEK-293 cells expressing mouse axin1.

Immunogen: synthetic peptide corresponding to amino acids 815-833 located near the C-terminal region of human axin1, conjugated to KLH. This sequence is identical in rat and mouse axin1 and highly conserved (83% identity) in human, rat, and mouse axin2.

Physical form: Solution in 0.01 mM phosphate buffered saline, pH 7.4, and 15 mM sodium azide.