• PRODUCT AVAILABILITY: Did you know you can view a product's availability right on the product page? Simply enter the quantity you want to purchase and the current availability will appear below the item.

  • Product C840L43 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

Jackson Immunoresearch

Rhodamine Red™-X (RRX) AffiniPure F(ab')2 Fragment Goat Anti-Human IgA + IgG + IgM (H+L)

Not yet rated

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with human IgA, IgG, and IgM. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Target: Human

Host: Goat

Antibody Format: F(ab')2 Fragment

Specificity: IgA + IgG + IgM (H+L)

Conjugate: Rhodamine Red™-X (RRX)

Clonality: Polyclonal

Physical StateFreeze-dried powder
StorageStore freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. 
Expiration dateone year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityThe antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. 
Buffer 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
Stabilizer15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
Preservative0.05% Sodium Azide 
Suggested Working Concentration or Dilution Range
Histo-/Cyto-chemistry- 1:50-1:200 
Flow Cytometry- 1:50-1:200 
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Please Enter Your Order Info

Filter by:

  • Clear Filters
Product Detail
Thomas No.
C840L43
Mfr. No.
109-296-064
Description
Rhodamine Red™-X (RRX) AffiniPure F(ab')2 Fragment Goat Anti-Human IgA + IgG + IgM (H+L), 1.0mg

list price/quantitytotal
$0.00
$0.00 (0 Items)
Product C840L43 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

Write A Review