Immunogen: Purified recombinant Sudan Ebola virus (SEBOV) Glycoprotein (GP), modified.
Description: Protein A purified rabbit polyclonal antibody reactive to SEBOV GP. The antibody detects recombinant SEBOV GP (modified) and GP expressed in virus-like particles (VLP) in Western blot and ELISA.
Supplied: 500 µg of antibody is supplied in PBS at a concentration of 1 mg/mL. 0.01% Sodium azide has been added
Raised in: Rabbits
Purification: Antibody was purified using a Protein A column.
Clonality: Polyclonal
Relevance: The antibody can be used for detection of SEBOV GP.
Recommended Dilutions:
ELISA: Assay-dependent dilution
WB: Assay-dependent dilution; internal QC demonstrates strong detection of 0.2 µg of purified recombinant SEBOV GP (modified) with a use dilution from 1 - 5 µg per mL of antibody in diluent using an HRP antibody conjugate and chromogenic substrate for visualization.
Storage: 2-3 weeks +4°C, -20°C long term.
Cross Reactivity: Western blotting demonstrates significant cross reactivity to Zaire Ebola Virus (ZEBOV) GP expressed in virus-like particles. A very slight cross-reactivity to Marburg virus (MARV) may also be observed. Cross reactivity to the HA epitope tag of the immunogen may also be observed.
Intended for research use only, not for human, therapeutic, or diagnostic applications.