Immunogen: Peptide sequence to Marburg virus (MARV) glycoprotein (GP); Sequence is specific to the GP2 subunit.
Description: Affinity purified rabbit polyclonal antibody reactive to MARV GP. The antibody detects GP in virus-like particles (VLP) and recombinant GP without the transmembrane region (rGPΔTM) in Western blot and ELISA.
Supplied: 100 µg of antibody is supplied in PBS at a concentration of 1.147 mg/mL. 0.02% Sodium azide has been added.
Raised in: Rabbits
Purification: Antibody is affinity purified using immobilized immunogen.
Relevance: The antibody can be used for detection of MARV GP and more specifically, MARV GP2 when the protein is denatured and reduced.
ELISA: Assay-dependent dilution
WB: Assay-dependent dilution; internal QC demonstrates detection of at least 0.2 µg of partially purified recombinant MARV rGPΔTM with a use dilution of 50 ng per mL of antibody in diluent using an peroxidase antibody conjugate and chromogenic substrate for visualization.
Storage: 2-3 weeks +4°C, -20°C long term.
Cross Reactivity: In Western blotting: No cross-reactivity was observed to Ebola virus (EBOV) or Sudan virus (SUDV) VLP or rGPΔTM. In ELISA: Slight reactivity against EBOV and SUDV VLP only at the highest concentration of antibody. No cross-reactivity was observed against EBOV and SUDV rGPΔTM.
Intended for research use only, not for human, therapeutic, or diagnostic applications.