Immunogen: Purified Panton-Valentine Leukocidin, Luk-S subunit, expressed in E.coli (His-tagged).
Description: Rabbit polyclonal reactive to Staphylococcus aureus Luk-S. The antibody detects the antigen produced by S. aureus strains in Western blot and ELISA and neutralizes the cytolytic activity of Panton Valentine Leukocidin (PVL : Luk-S+Luk-F).
Size: 0.5mg at a concentration of 5.5 mg/mL
Buffer: PBS + 0.01% Sodium Azide
Raised in: New Zealand White Rabbits
Purification: Protein A
Relevance: The antibody can be used for detection and neutralization of PVL Luk-S produced by PVL-producing virulent strains of S. aures such as CA-MRSA USA300.
ELISA: Assay-dependent dilution
WB: Assay-dependent dilution; if probing against purified protein, at least 10 ng of pure toxin can be detected with 0.5 ug/ml of the primary antibody combined with a suitable dilution of a secondary anti-rabbit antibody.
Toxin neutralization: Using a neutrophil lysis assay, 62.5 μg/ml of this antibody inhibits 100% of the lytic activity of 0.1 μg/ml of PVL.
IF: Not tested
Storage: 2-3 weeks +4°C, -20°C long term
Species Reactivity: unknown.
Intended for research use only, not for human, therapeutic, or diagnostic applications.