Immunogen: Purified N-terminal domain of Staphylococcus aureus alpha hemolysin expressed in E.coli (His-tagged).
Description: Rabbit polyclonal reactive to S. aureus alpha hemolysin (α-toxin). The antibody detects the antigen produced by S. aureus strains in western blot and ELISA and neutralizes the hemolytic activity of the toxin.
Buffer: PBS + 0.01% Sodium Azide
Raised in: New Zealand White Rabbits
Purification: Protein A
Relevance: The antibody can be used for detection and neutralization of α-toxin produced by virulent strains of S. aureus.
ELISA: Assay-dependent dilution
WB: Assay-dependent dilution; if probing against purified protein, at least 25 ng of pure α-toxin can be detected with 1 ug/ml of the primary antibody combined with a suitable dilution of a secondary anti-rabbit antibody.
Toxin neutralization: using a rabbit RBC lysis assay, IC50 of this antibody for neutralization of 1 ug/ml of α-toxin was determined to be 14 ug/ml.
IF: Not tested
Storage: 2-3 weeks +4°C, -20°C long term
Species Reactivity: unknown.
Intended for research use only, not for human, therapeutic, or diagnostic applications.