Immunogen: Sucrose purified virus from infected supernatant; CHIKV 181/25
Description: Rabbit polyclonal for CHIKV strain 181/25 purified from antiserum using a Protein G column
Size: 0.5 mg is supplied in PBS at a concentration of 5 mg/mL. No preservative has been added.
Raised in: New Zealand White Rabbits
Purification: Antiserum was purified using a Protein G column
Clonality: Polyclonal
Relevance: The 181/25 vaccine strain represents a surrogate strain for the Category C (NIAID) virus Chikungunya.
Recommended Dilutions:
ELISA: Assay-dependent dilution
WB: Assay-dependent dilution; QC testing demonstrates strong signal with the antibody between 1.0-0.1 µg/mL. If probing against purified infected cell supernatant, cross-adsorption is recommended.
IF: Antiserum prior to IgG purification was utilized at a 1:500 dilution with Cy3 goat anti-rabbit secondary. The purified antibody has not been tested but can be optimized accordingly.
Storage: It is recommended that aliquots be made and stored at -20°C long term; if storing at 4°C is desired longer term, it is recommended that a preservative be added.
Species Reactivity: Some cross-reactivity is observed with CHIKV OPY-1, CHIKV S27, fetal bovine serum, Vero cells, and Venezuelan Equine Encephalitis strain TC-83. The antibody does not react with Vaccinia NYCBOH, Junin Candid #1, or Rift Valley Fever MP12.
Intended for research use only, not for human, therapeutic, or diagnostic applications.