Product is the lyophilized powder of rabbit IgG fraction to hamster IgG (whole molecule) and buffer salts. The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining.
SUGGESTED WORKING DILUTIONS
- 1:50 - 1:200 for Tissue Staining
- 1:100 - 1:500 for Immunoblotting
- 1:400 - 2,000 for Elisa
It is recommended that each lab obtain their own optimal working dilution for each assay
Reconstitute product with 2.0 ml of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Store lyophilized product at 2-8°C. For long term storage, aliquot and store reconstituted product frozen at -20°C or lower.
SPECIFICATIONS: The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/ml using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected. It shows reactivity to hamster IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG, some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.