The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized with an in-house control by immunoelectrophoresis. Each antiserum is tested for specificity at a minimum of 80 mg/mL using immunoelectrophoresis.
The antiserum is prepared by hyperimmunizing rabbits with human seminal fluid; antiserum is delipidated and made specific by solid-phase absorption. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
Rabbit Antiserum to Human Seminal Fluid is used as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.
Product is the lyophilized powder of rabbit antiserum to human seminal fluid (absorbed against human serum) and buffer salts.
Concentration: Total Protein: 34.4 mg/mL
Key Applications: Precipitating Antibody
Application Areas: Immunoassays
Research Areas: Cell Biology
Product Type: Antibodies
Antibody Type: Primary Antibodies
Antibody Category: Polyclonal
Species Reactivity: It shows reactivity to human seminal fluid; cross-reactivity to other species may exist. Antibody activity to serum proteins is not present.
NOTES: Ship: cold packs
Solubility: Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Storage & Handling: Store lyophilized product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage, aliquot and freeze reconstituted product at -20°C or lower.