Synonyms: anti-green fluorescent protein
Purity: >90% (HPLC)
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Features and Benefits:
Mixture of two monoclonal antibodies, supplied as a white lyophilizatecontaining 200μg of total Anti-GFP IgG.
Anti-GFP is a mixture of two clones (7.1 and 13.1).
Monoclonal antibody for detection of GFP or GFP fusions using:
• Western blots
Mixture of two high-affinity monoclonal antibodies selected for their performance in detection of GFP and GFP fusion proteins.
For life science research only. Not for use in diagnostic procedures.
Anti-GFP is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation.
Purity: Both Anti-GFP mouse monoclonal antibodies (Clones 7.1 and 13.1) are >95% pure as determined by SDS-PAGE and ion-exchange HPLC analyses.
Working concentration: Working concentration of antibody depends on application and substrate.
The following concentrations should be taken as a guideline:• Western blot: 1:1000 dilution
• Immunoprecipitation: 2 to 10 μg
Storage conditions (working solution): -15 to -25 °C
Add 500 μl double distilled water to a final concentration of 0.4 mg/ml.
Rehydrate on ice for 30 minutes.
Subtype: Both clones are Mouse IgG1κ
This product is sold under license from Columbia University. Rights to use this product are limited to research use only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of this product for commercial purposes should be directed to Columbia Innovation Enterprise, Columbia University, Engineering Terrace - Suite 363, new York, New York 10027.