Synonyms: anti-green fluorescent protein
Purity: >90% (HPLC)
Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Features and Benefits:
Contents
Mixture of two monoclonal antibodies, supplied as a white lyophilizatecontaining 200μg of total Anti-GFP IgG.
Anti-GFP is a mixture of two clones (7.1 and 13.1).
Application:
Monoclonal antibody for detection of GFP or GFP fusions using:
• Immunoprecipitation
• Western blots
• Immunostaining
General description:
Mixture of two high-affinity monoclonal antibodies selected for their performance in detection of GFP and GFP fusion proteins.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Anti-GFP is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation.
Purity: Both Anti-GFP mouse monoclonal antibodies (Clones 7.1 and 13.1) are >95% pure as determined by SDS-PAGE and ion-exchange HPLC analyses.
Preparation Note:
Working concentration: Working concentration of antibody depends on application and substrate.
The following concentrations should be taken as a guideline:• Western blot: 1:1000 dilution
• Immunoprecipitation: 2 to 10 μg
Storage conditions (working solution): -15 to -25 °C
Reconstitution:
Add 500 μl double distilled water to a final concentration of 0.4 mg/ml.
Rehydrate on ice for 30 minutes.
Specificity:
Subtype: Both clones are Mouse IgG1κ
Legal Information:
This product is sold under license from Columbia University. Rights to use this product are limited to research use only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of this product for commercial purposes should be directed to Columbia Innovation Enterprise, Columbia University, Engineering Terrace - Suite 363, new York, New York 10027.