Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Analysis Note:
No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.
Application:
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using:
• ELISA
• Immunohistocytochemistry
• In situ hybridization
• Western blot
General description:
Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Preparation Note:
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline: • ELISA: for coating: 2 to 4 μg/ml
• Immunohistocytochemistry: 0.5 to 2 μg/ml
• In situ hybridization: 0.2 to 0.4 μg/ml
• Western blot: 0.5 to 2 μg/ml
Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
Reconstitution:
Add 1 ml PBS to a final concentration of 200 μg/ml.
Specificity:
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.