Product is horseradish peroxidase (HRP)-conjugated rabbit affinity purified antibody to goat IgG (whole molecule) and buffer salts.
Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. No preservative is added.
This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. See Related Product Ordering Information for these products.)
Suggested Working Dilutions:
1:200 - 1:800 for Tissue Staining
1:500 - 1:2,000 for Immunoblotting
1:1,000 - 4,000 for Elisa
It is recommended that each lab obtain their own optimal working dilution for each assay.
Reconstitute product with 2.0 ml of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material.
Store product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long term storage, aliquot and store with 40% glycerol (v/v) at -20°C.
The total protein is measured using the Biuret procedure with bovine albumin as standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with goat IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is rabbit IgG; no trace of albumin is detected. It shows reactivity to goat IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG, some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.