Preparation Method
Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. No preservative is added.
Applications
This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.)
Typical Working Concentration
SUGGESTED WORKING DILUTIONS: 1:200 - 1:800 for Tissue Staining 1:500 - 1:2,000 for Immunoblotting 1:1,000 -1: 4,000 for Elisa