The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to non-immunoglobulin serum proteins is not present.
The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (whole molecule) antibody recognizes IgG1, G2a, G2b, and G3, mouse IgM and IgA. The identity and purity of affinity isolated antibody is established by immunoelectrophoresis.
Goat IgG fraction to mouse IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. The antibody was used in ELISA and RIA in various studies. It is also suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).
Product is the lyophilized powder of goat IgG fraction to mouse IgG (whole molecule) and buffer salts.
Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Concentration: Total Protein: 24.6 mg/mL; Antibody Protein: 10 mg/mL
Key Applications: Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections
Application Areas: Immunoassays
Product Type: Antibodies
Antibody Type: Secondary Antibodies
Antibody Category: Polyclonal
Host: Goat
Immunogen: Pooled normal mouse serum
Species Reactivity: It shows reactivity to mouse IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG, some antibodies are expected to bind light chain sites common to all immunoglobulins.
Format: Lyophilized IgG
Storage & Handling: Lyophilized product stored at 2-8°C has a shelf life of at least five years. After reconstitution, product will retain activity for at least 6 months stored at 2-8°C. For long term storage of up to two years, aliquot and store reconstituted product frozen at -20°C or lower.
Certifications: The product is mostly goat IgG.