Antibodies are produced in rabbits by immunization with feline immunoglobulins and formulated with selected beta globulins including the C3 component of complement. The resulting antiserum is heated at 56°C for 30 minutes to inactivate rabbit complement. Heteroagglutinins are absorbed with pooled normal feline red blood cells. The reagent is tested with normal and antibody-coated feline red blood cells to assure its specificity.
Autoimmune hemolytic anemia (AIHA) is an autoimmune disease characterized by antibody coated red blood cells that either lyse in the presence of complement, or are subject to phagocytosis by the liver and spleen, resulting in a severe anemia.
Feline anti-globulin reagent (Coombs') has been widely used for the diagnosis of autoimmune hemolytic anemia (AIHA) in a variety of species. Several disease entities resembling AIHA and immune-mediated hemolytic anemia have been recognized in the cat. Feline antiglobulin test can be used as a test to aid the diagnosis of AIHA and immune-mediated hemolytic anemia in the cat.
Reconstitute with 2.0 mL of sterile distilled water.
Key Applications: Autoimmune Hemolytic Anemia (AIHA) diagnosis
Application Areas: Immunoassays
Research Areas: Autoimmune Diseases
Product Type: Antibodies
Antibody Type: Primary Antibodies
Host: Rabbit
Presentation: Feline antiglobulin reagent is presented in the lyophilized form.
Format: Lyophilized antiserum
NOTES: Anti-Feline Globulins- IgG, C3
Storage & Handling: The lyophilized product has a minimum shelf life of five years from the manufacture date when stored at 2-8°C. After reconstitution, aliquot and keep frozen at or below -20°C for up to one year. Avoid repeated freeze/thaw cycles. Discard the reagent when it appears turbid or otherwise visibly contaminated.
Disease Screened: Autoimmune hemolytic anemia (AIHA)