Antibodies are produced in rabbits by immunization with equine immunoglobulins and formulated with selected β globulins including the C3 component of complement. The resulting antiserum is heated at 56°C for 30 minutes to inactivate rabbit complement. Heteroagglutinins are absorbed with pooled normal equine red blood cells. The reagent is tested with normal and antibody-coated equine red blood cells to assure its specificity.
Autoimmune hemolytic anemia (AIHA) is an autoimmune disease characterized by antibody coated red blood cells that either lyse in the presence of complement, or are subject to phagocytosis by the liver and spleen, resulting in a severe anemia.
Equine anti-Globulin reagent has been widely used for the diagnosis of autoimmune hemolytic anemia (AIHA) in a variety of species. Although not common AIHA has been reported in the horse. Equine anti-globulin reagent is also useful in the neonatal isoerythrolysis test. The anti-globulin procedure has also been used in equine blood typing.
Key Applications: Autoimmune Hemolytic Anemia (AIHA) diagnosis
Application Areas: Immunoassays
Research Areas: Autoimmune Diseases
Product Type: Antibodies
Antibody Type: Primary Antibodies
Host: Rabbit
Presentation: Equine anti-globulin reagent is presented in the lyophilized form.
Format: Lyophilized antiserum
NOTES: Anti-Equine Globulins- IgG, C3
Storage & Handling: Store the lyophilized product at 2-8°C. After reconstitution, aliquot and keep frozen at or below -20°C. Avoid repeated freeze/thaw cycles. Discard the reagent when it appears turbid or otherwise visibly contaminated.
Disease Screened: Autoimmune hemolytic anemia (AIHA)