Polyclonal antibody for CTLA 4/Ctla4 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: ELISA. Reactive species: Mouse. CTLA 4/Ctla4 information: Molecular Weight: 24993 MW; Subcellular Localization: Cell membrane ; Single-pass type I membrane protein ; Tissue Specificity: Widely expressed with highest levels in lymphoid tissues.
Background:
CTLA-4(Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that down regulates the immune system. It is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.
Attributes:
sku: A00020-1
name: Anti-CTLA4 Picoband™ Antibody
gene_name: Ctla4
clonality: Polyclonal
concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
size: 100ug/vial
uniprot_id: P09793
host: Rabbit
immunogen: E. coli-derived mouse CTLA4 recombinant protein (Position: E36-D161). Mouse CTLA4 shares 67.5% amino acid (aa) sequence identity with human CTLA4.
form: Lyophilized
purification: Immunogen affinity purified.
storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
cross_reactivity: No cross reactivity with other proteins.
reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
application_details: Western blot, 0.1-0.5μg/ml, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
ELISA , 0.1-0.5μg/ml, Mouse
applications: ELISA, IHC-P, WB
reactivity: Mouse, Rat
research_category: T Cells, T Lymphocytic Lineage
synonyms: Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4;CTLA-4;CD152;Ctla4;Cd152;
gene_full_name: Cytotoxic T-lymphocyte protein 4
molecular_weight: 24993 MW
protein_function: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 (By similarity). .
subcellular_localization: Cell membrane ; Single-pass type I membrane protein .
tissue_specificity: Widely expressed with highest levels in lymphoid tissues. .
recommended_detection_systems: Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).