Gentamicin is an aminoglycoside antibiotic complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is a mixture of 3 major components designated as C1, C1a, and C2. Gentamicin is used as the sulfate salt. Each component consists of five basic nitrogens and requires five equivalents of sulfuric acid per mole of gentamicin base.
Gentamicin sulfate is a broad spectrum antibiotic. It inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms, including strains resistant to tetracycline, chloramphenicol, kanamycin, and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus, and Streptococcus. Gentamicin sulfate inhibits bacterial protein biosynthesis by binding to the 30S subunit of the ribosome.
Mode of action: Gentamicin causes codon misreading by binding to the 30S ribosomal subunit, blocking the translocation of peptidyl-tRNA from the acceptor site to the donor site.The bactericidal effect of gentamicin on Pseudomonas aeruginosa is exerted by the binding of gentamicin to the outer membrane, where it displaces natural cations, destabilizes the membrane, and forms holes in the cell surface. Antimicrobial spectrum: Gram-negative bacteria, Staphylococcus aureus and other Gram-positive bacteria.
Grade: Cell Culture Reagent
Potency: >590 µg/mg
Product Type: Biochemicals
Biochemical Category: Antibiotics
Melting Point: 218-237°C (Lit)
Optical Rotation: +116°
Presentation: White Powder
pH: 3.5-5.5 (4% solution in water (Lit.)
Solubility: Gentamicin sulfate is soluble in water (50 mg/mL), yielding a clear to very slightly hazy, colorless to faint. It is practically insoluble in alcohol and other organic solvents.
Storage & Handling: Store at +4°C.