Gentamicin Sulfate Reagent Solution is recommended for use in cell culture applications at 5 ml/L and used as a selection agent (gentamicin-resistance gene) in molecular biology applications.
Gentamicin is an aminoglycoside antibiotic complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is a mixture of 3 major components designated as C1 (C21H43N5O7), C1a (C19H39N5O), and C2 (C20H41N5O7). Gentamicin is used as the sulfate salt. Each component consists of five basic nitrogens and requires five equivalents of sulfuric acid per mole of gentamicin base. Gentamicin sulfate is a broad spectrum antibiotic. It inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms, including strains resistant to tetracycline, chloramphenicol, kanamycin, and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus, and Streptococcus. It is effective against mycoplasma as well.
Gentamicin Sulfate inhibits protein synthesis by binding to L6 protein of 50S ribosomal subunit. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria, and mycoplasma.
Concentration: 10 mg/mL
Potency: ≥590 µg/mg (dry basis), ≥483.8 µg/mg (as is)
Suitability: Suitable for cell culture
Product Type: Biochemicals
Chemical Class: Antibiotics
Presentation: Clear Colorless Solution
pH: 4.0 - 6.0
NOTES: Osmolality: 29 mM/kg (Lit.)
Storage & Handling: Store at +4°C.