Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein.
Poly-L-lysine is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When adsorbed to the culture surface, poly-lysine increases the number of positively charged sites available for cell binding. Can be used as a coating substrate since poly-lysine is a nonspecific attachment factor for cells; however, certain cells can digest poly-lysine. Other uses for poly-lysine have been reported as follows:
- Conjugation to methotrexate for increased drug transport
- Microencapsulation of islets
- Use in simple reproducible procedure for chromosomal preparations from a variety of tissues
- Immobilation of living cells in biocompatible semipermeable microcapsules
- Poly-L-Lysine In the preparation of polycationic beads
- Conjugation to albumin and horseradish peroxidase to enhance cellular uptake
Typically, the effective coating solution concentration for culture dishes will be 0.3 to 0.5% and for histology slides at 0.1%. Recommended as a cell culture substratum when using 0.5 mL of a 0.1 mg/mL solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.
Grade: Cell culture reagent, y-Irradiated
Suitability: suitable for cell culture
Key Applications: Cellular attachment factor in culture applications
Product Type: Biochemicals
Presentation: Cream Powder
NOTES: There is approximately one HBr per lysine unit. The HBr allows it to be a crystalline solid and soluble in water. To remove the HBr, solubilize the product in a neutral buffer and dialyze away the salts.
Solubility: Soluble in 50 mL H2O/vial.
Storage & Handling: -20°C.