UNSPSC Code: 41115700
RIDADR: NONH for all modes of transport
Recovery of labeled RNA: >80% (poly [A], approximately 500 bases)
Retention of unincorporated mononucleotides: >99%
Exclusion limit for RNA: <72 bases
Sample size: up to 100 μl
Time required: 10 minutes
Remove unincorporated precursors from DNA labeled by nick translation, end labeling, polymerization reactions, and other labeling techniques.
Use the highly purified radiolabeled DNA in Southern blotting and other downstream applications.
Quick Spin Columns are produced with material shown to have a ≥80% RNA recovery.
All the tedious, time-consuming steps involved in preparing columns have already been performed. Quick Spin Columns are pre-packed, pre-swollen, and quality tested to ensure:• maximal retention of unincorporated nucleotides (≥99%)
• absence of DNase and RNase contamination according to the current Quality Control procedures.
For life science research only. Not for use in diagnostic procedures.
Quick Spin columns contain gel filtration matrices which allow large molecules (e.g., DNA or RNA) to pass through quickly while retaining small molecules (e.g., nucleotides). The Quick Spin format improves the molecular sieving concept by using centrifugation to separate DNA or RNA rapidly and cleanly from small contaminants.