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MilliporeSigma

ROCHE Quick Spin Columns for radiolabeled RNA purification, Sephadex G-50, fine

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Storage: 2-8°C

UNSPSC Code: 41115700

RIDADR: NONH for all modes of transport

Analysis Note:
Recovery of labeled RNA: >80% (poly [A], approximately 500 bases)
Retention of unincorporated mononucleotides: >99%
Exclusion limit for RNA: <72 bases
Sample size: up to 100 μl
Time required: 10 minutes

Application:
Remove unincorporated precursors from DNA labeled by nick translation, end labeling, polymerization reactions, and other labeling techniques.
Use the highly purified radiolabeled DNA in Southern blotting and other downstream applications.

General description:
Quick Spin Columns are produced with material shown to have a ≥80% RNA recovery.
All the tedious, time-consuming steps involved in preparing columns have already been performed. Quick Spin Columns are pre-packed, pre-swollen, and quality tested to ensure:• maximal retention of unincorporated nucleotides (≥99%)
• absence of DNase and RNase contamination according to the current Quality Control procedures.

Other Notes:
For life science research only. Not for use in diagnostic procedures.

Principle:
Quick Spin columns contain gel filtration matrices which allow large molecules (e.g., DNA or RNA) to pass through quickly while retaining small molecules (e.g., nucleotides). The Quick Spin format improves the molecular sieving concept by using centrifugation to separate DNA or RNA rapidly and cleanly from small contaminants.

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Product Detail
Thomas No.
C756Z59
Mfr. No.
11274015001
Description
Quick Spin Columns for radiolabeled RNA purification, pkg of 20 columns
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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