Description: Recombinant TSST-1 expressed in E.coli and purified to >90% using chromatographic methods.
Size: 100 µg of protein is supplied in PBS + 10% Glycerol at a concentration of 1.3 mg/mL. The theoretical molecular weight of the TSST-1 is 22,204 Daltons.
Relevance: TSST-1 is a pyrogenic toxin superantigen secreted by Staphylococcus aureus. TSST-1 is a powerful activator of T-lymphocytes resulting in substantial cytokine release that can cause severe pathological consequences.
rTSST-1 can be utilized as a control in ELISA and Western blot assays, as a potent mitogen, or as an activator of cytokines in Peripheral Blood Mononuclear Cells (PBMC).
Recommended Dilutions:
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution; internal QC demonstrates strong detection of 1 ng of TSST-1.
PBMC Stimulation assay: Stimulation of PBMCs to produce Interferon γ by TSST-1 can be achieved with 0.1 ng/mL.
Storage: 2-3 weeks at -20°C, -80°C long term
Intended for research use only, not for human, therapeutic, or diagnostic applications.