Description: : Purified, Staphylococcus aureus recombinant leukocidin-E (rLukE). The rLukE (tag free) is expressed in E. coli and purified by FPLC. The theoretical molecular weight of the protein is 34,819 Daltons
Size: 100 µg of protein is supplied in PBS at a concentration of 2.417 mg/mL. Protein demonstrates a molecular weight of approximately 35 kDa.
Relevance: This protein may be used in functional toxicity assays in combination with rLukD, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus.
Recommended Dilutions:
ELISA: Assay-dependent dilution
WB: Assay-dependent dilution; internal QC demonstrates detection of 100 ng of rLukE protein using anti-LukS-PV polyclonal antibody (cat# 04-0009) by Western blot analysis.
Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with rLukD in a concentration range of 25-800 nM.
Storage: 2-3 weeks at -20°C, -80°C long term.
Intended for research use only, not for human, therapeutic, or diagnostic applications.