Description: Purified, Staphylococcus aureus recombinant Gamma Hemolysin A (Hlg A). The rHlg A (tag free) is expressed in E. coli and purified by FPLC. The theoretical molecular weight of the protein is 34,956 Daltons.
Size: 100 ug of protein is supplied in PBS at a concentration of 2.065 mg/mL. Protein demonstrates a molecular weight of approximately 35 kDa.
Endotoxin: 3.332 Endotoxin Units/mg
Relevance: This protein may be used in functional toxicity assays in combination with rHlg B, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus.
Recommended Dilutions:
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution; internal QC demonstrates detection of 50 ng of rHlg A protein using anti-PVL LukS polyclonal antibody (cat# 04-0009) in Western blotting
Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with rHlg B in a concentration range of 4-200 nM.
Storage: 2-3 weeks at -20°C, -80°C long term
Intended for research use only, not for human, therapeutic, or diagnostic applications.